| 250 - 499 | Number of employees | Specialization
|
|---|
| Foxconn Global Services Division s.r.o. | 484 | Management consultancy |
| Globus ČR, k.s. | 477 | Retail |
| RONAL CR s.r.o. | 468 | Manufacture of alloy wheels |
| Chládek a Tintěra, Pardubice a.s. | 453 | Civil engineering |
| Dopravní podnik města Pardubic a.s. (Public Transport Company of Pardubice City) | 451 | Transport |
| City of Pardubice | 437 | Public administration |
| International Power Opatovice, a.s. (Opatovice nad Labem) | 426 | Production of electricity and district heating |
| JTEKT Automotive Czech Pardubice s.r.o. | 384 | Automobile industry |
| Pardubický kraj (Pardubice Regional Authority) | 366 | Public administration |
| BV elektronik s.r.o. (Holice) | 350 | Electronics |
| APM Global Logistics Czech Republic, s.r.o. | 315 | doprava - služby v dopravě |
| Sociální služby města Pardubic (Social Services of Pardubice City) | 314 | Social services |
| CZECH - XAWAX s.r.o. | 286 | Recruitment agency |
| Telefónica O2 Czech Republic, a.s. | 277 | Communications |
| České dráhy, a.s. - Czech Rails - Česká Třebová Railway Vehicle depot, Pardubice Branch Office | 275 | Transport |
| Léčebné lázně Bohdaneč a.s. (Lázně Bohdaneč Peat Spa)) | 273 | Spa |
| Česká pojišťovna a.s. (Czech Insurance Company) | 267 | Financial services and insurance |
| Erwin Junker Grinding Technology a.s. (Holice) | 265 | Engineering |
| KYB Manufacturing Czech s.r.o. | 256 | Automobile industry |
| ČEZ, a. s. (Chvaletice) | 256 | Production of electricity |
| Nedcon Bohemia, s.r.o. | 255 | Engineering |
| Správa železniční dopravní cesty, státní organizace (State Organization for Management of Railways) | 254 | Transport |
| Výzkumný ústav organických syntéz a.s. (Rybitví) (Research Institute of Organic Syntheses) | 251 | Chemical industry |
| ERA a.s. | 250 | Electrical engineering |